PRJNA739877

BioProject first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Mus musculus amplicon sequencing via NovaSeq 6000 from 11 samples totaling 18.8 billion bases (97.1% Q20), supporting high-throughput mouse targeted profiling with excellent per-sample quality.

Data type / assay

amplicon

Organism

Mus musculus

Instrument

Illumina NovaSeq 6000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

11 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 18849979800 reported

total reads 125666532 reported

n content pct 0.003 measured

pct q20 bases 97.1 measured

pct q30 bases 92.8 measured

gc content pct 60.1 measured

mean read length 150 measured

mean base quality 35.7 measured

adapter content pct 0.01 measured

duplication rate pct 95.51 measured

mean target coverage 349.1 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 92.8 measured ×1 100%

adapter content pct 0.01 measured ×0.5 100%

QC cost 58 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0