PRJNA754792

BioProject first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

95/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

NextSeq 550 deep amplicon sequencing of adeno-associated virus (AAV) constructs with good quality (94.3% Q20) and very high read depth (~294 million reads) enables viral library characterization and variant mapping in gene therapy applications.

Data type / assay

amplicon

Organism

Adeno-associated virus

Instrument

NextSeq 550

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

56 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 21806092605 reported

total reads 294224450 reported

n content pct 0.001 measured

pct q20 bases 94.3 measured

pct q30 bases 88.6 measured

gc content pct 57.1 measured

mean read length 72.1 measured

mean base quality 33.6 measured

adapter content pct 0.01 measured

duplication rate pct 4.82 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 95/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 88.6 measured ×1 93%

adapter content pct 0.01 measured ×0.5 100%

QC cost 33 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0