PRJNA758283

BioProject first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

79/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Mouse bulk RNA-seq from an Illumina HiSeq 2500 with 306 million short reads at 94% Q30, totaling 92 billion bases. This very deep transcriptome enables detection of tissue-specific and condition-specific transcripts with high precision.

Data type / assay

bulk-RNA-seq

Organism

Mus musculus

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

12 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 92099483700 reported

total reads 306998279 reported

n content pct 0.004 measured

pct q20 bases 97.8 measured

pct q30 bases 94.4 measured

gc content pct 50 measured

mean read length 150 measured

mean base quality 39 measured

adapter content pct 16.6 measured

duplication rate pct 50.88 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 79/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.4 measured ×1 100%

mean base quality 39 measured ×0.6 100%

adapter content pct 16.6 measured ×0.4 19%

duplication rate pct 50.88 measured ×0.4 54%

QC cost 30 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0