Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA773399

BioProject first seen 2022

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

17/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

SARS-CoV-2 bulk RNA-seq via NextSeq 550 from 54 samples reaching 106 billion bases; however, quality is severely compromised with only 17.1% Q20 and 7.8% GC content, indicating massive rRNA contamination or protocol failure rendering data unsuitable for variant calling or quantitation.

Data type / assay
bulk-RNA-seq
Organism
Severe acute respiratory syndrome coronavirus 2
Instrument
NextSeq 550
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
54 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 106124171734 reported
total reads 671671973 reported
n content pct 81.761 measured
pct q20 bases 17.1 measured
pct q30 bases 16.4 measured
gc content pct 7.8 measured
mean read length 127 measured
mean base quality 6.2 measured
adapter content pct 0.12 measured
duplication rate pct 87.89 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 17/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 16.4 measured ×1 0%
mean base quality 6.2 measured ×0.6 0%
adapter content pct 0.12 measured ×0.4 100%
duplication rate pct 87.89 measured ×0.4 0%
QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0