Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA779049

BioProject first seen 2024

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

44/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Mus musculus bulk RNA-seq from HiSeq 2000 across 12 samples generating 437 billion bases (78.6% Q20), providing substantial transcriptome depth despite notably lower quality metrics (61.8% Q30) that may indicate systematic sequencing or sample issues requiring careful quality filtering.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
12 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 437456617800 reported
total reads 1458188726 reported
n content pct 0 measured
pct q20 bases 78.6 measured
pct q30 bases 61.8 measured
gc content pct 33.5 measured
mean read length 150 measured
mean base quality 30.5 measured
adapter content pct 0.05 measured
duplication rate pct 9.32 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 44/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 61.8 measured ×1 0%
mean base quality 30.5 measured ×0.6 42%
adapter content pct 0.05 measured ×0.4 100%
duplication rate pct 9.32 measured ×0.4 100%
QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0