PRJNA808399

BioProject first seen 2022

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

75/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

NextSeq 500 short-read RNA-seq in human samples with clean data (0.002% N-content) and moderate base quality (86.6% Q20); delivers ~157 million reads sufficient for gene expression quantification in mid-scale transcriptomic studies.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

20 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 13495916446 reported

total reads 156929261 reported

n content pct 0.002 measured

pct q20 bases 86.6 measured

pct q30 bases 82.4 measured

gc content pct 43.2 measured

mean read length 86 measured

mean base quality 31.9 measured

adapter content pct 1.62 measured

duplication rate pct 27.96 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 75/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.4 measured ×1 62%

mean base quality 31.9 measured ×0.6 65%

adapter content pct 1.62 measured ×0.4 100%

duplication rate pct 27.96 measured ×0.4 100%

QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0