PRJNA81021

BioProject first seen 2012

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Legacy 454 GS FLX Titanium pyrosequencing amplicon from environmental samples with 24K reads and 95.7% Q20/Q30, representing older technology with modest throughput. Useful for historical method comparison or re-analysis of legacy environmental sequence datasets.

Data type / assay

amplicon

Organism

environmental samples

Instrument

454 GS FLX Titanium

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

2 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 8431386 reported

total reads 24260 reported

n content pct 0.002 measured

pct q20 bases 95.7 measured

pct q30 bases 95.7 measured

gc content pct 55.9 measured

mean read length 307.2 measured

mean base quality 28.8 measured

adapter content pct 0 measured

duplication rate pct 97.59 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.7 measured ×1 100%

adapter content pct 0 measured ×0.5 100%

QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0