PRJNA81579

BioProject first seen 2013

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

34/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Japygidae (hexapod) EST library from a 454 GS FLX Titanium platform with 848k long reads at 66% Q30. Though modest in scale, this transcriptome survey captures expressed genes from an understudied arthropod lineage useful for comparative genomics.

Data type / assay

bulk-RNA-seq

Organism

Japygidae

Instrument

454 GS FLX Titanium

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 346322935 reported

total reads 848407 reported

n content pct 0.108 measured

pct q20 bases 84.7 measured

pct q30 bases 66.3 measured

gc content pct 48.1 measured

mean read length 289.8 measured

mean base quality 31.5 measured

adapter content pct 0 measured

duplication rate pct 66.96 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 34/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 66.3 measured ×1 0%

mean base quality 31.5 measured ×0.6 58%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 66.96 measured ×0.4 18%

QC cost 14 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0