PRJNA81581

BioProject first seen 2013

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

25/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Thermobia domestica (firebrat) EST sequencing via 454 GS FLX Titanium generating 455 million bases (80.7% Q20), capturing expressed sequence tags for this basal hexapod; lower read quality (57.6% Q30) and modest depth limit transcript discovery compared to modern RNA-seq.

Data type / assay

bulk-RNA-seq

Organism

Thermobia domestica

Instrument

454 GS FLX Titanium

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 454804493 reported

total reads 821535 reported

n content pct 0.063 measured

pct q20 bases 80.7 measured

pct q30 bases 57.6 measured

gc content pct 35.8 measured

mean read length 338.9 measured

mean base quality 29.6 measured

adapter content pct 0 measured

duplication rate pct 71.09 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 25/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 57.6 measured ×1 0%

mean base quality 29.6 measured ×0.6 27%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 71.09 measured ×0.4 9%

QC cost 38 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0