PRJNA846135

BioProject first seen 2023

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Illumina HiSeq 2500 amplicon of rat gut microbiota with 1.2M reads and exceptional quality (98.2% Q20, 96.6% Q30) but modest total bases, suited for species-level identification with high confidence. Limited depth appropriate for strain tracking and rare taxon surveys.

Data type / assay

amplicon

Organism

rat gut metagenome

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

18 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 675003828 reported

total reads 1228432 reported

n content pct 0 measured

pct q20 bases 98.2 measured

pct q30 bases 96.6 measured

gc content pct 51.9 measured

mean read length 276.2 measured

mean base quality 38.1 measured

adapter content pct 0 measured

duplication rate pct 98.6 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.6 measured ×1 100%

adapter content pct 0 measured ×0.5 100%

QC cost 5 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0