PRJNA872285

BioProject first seen 2022

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Mouse gut metagenome amplicon sequencing via HiSeq 2500 across 12 samples totaling 447 million bases (97.6% Q20), enabling microbiome profiling at modest depth; amplicon approach targets specific bacterial regions suitable for taxonomic composition analysis.

Data type / assay

amplicon

Organism

mouse gut metagenome

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

12 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 446724931 reported

total reads 1015286 reported

n content pct 0 measured

pct q20 bases 97.6 measured

pct q30 bases 92.8 measured

gc content pct 52.6 measured

mean read length 220 measured

mean base quality 35.8 measured

adapter content pct 0 measured

duplication rate pct 98.01 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 92.8 measured ×1 100%

adapter content pct 0 measured ×0.5 100%

QC cost 5 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0