PRJNA970328

BioProject first seen 2023

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

76/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Mus musculus bulk RNA-seq via NovaSeq 6000 from 18 samples reaching 344 billion bases with outstanding quality (99.9% Q20, 99.9% Q30), enabling sensitive mouse transcriptomics across multiple biological conditions; extremely high quality reflects optimized sequencing run conditions.

Data type / assay

bulk-RNA-seq

Organism

Mus musculus

Instrument

Illumina NovaSeq 6000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

18 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 344300022900 reported

total reads 1147666743 reported

n content pct 0.001 measured

pct q20 bases 99.9 measured

pct q30 bases 99.9 measured

gc content pct 49.1 measured

mean read length 150 measured

mean base quality 30 measured

adapter content pct 9.63 measured

duplication rate pct 29.88 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 76/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 99.9 measured ×1 100%

mean base quality 30 measured ×0.6 33%

adapter content pct 9.63 measured ×0.4 58%

duplication rate pct 29.88 measured ×0.4 100%

QC cost 44 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0