SRP000109

ENA first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

454 GS 20 amplicon sequencing of marine metagenome with moderate quality (91.2% Q20/Q30) and 41% GC content; produces reads suitable for 16S/18S taxonomic profiling of marine microbial communities despite the legacy platform's read-length limitations.

Data type / assay

amplicon

Organism

marine metagenome

Instrument

454 GS 20

Platform

CAPILLARY

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

87 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 28419471659 reported

total reads 56579057 reported

n content pct 1.055 measured

pct q20 bases 91.2 measured

pct q30 bases 91.2 measured

gc content pct 41 measured

mean read length 114.2 measured

mean base quality 27.6 measured

adapter content pct 0 measured

duplication rate pct 5.56 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91.2 measured ×1 100%

adapter content pct 0 measured ×0.5 100%

QC cost 3 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0