SRP001899

ENA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

54/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Apis mellifera bulk RNA-Seq (EST) on 454 GS FLX with 1.36M reads at moderate quality (87.7% ≥Q20, 70.7% ≥Q30). Legacy platform honeybee transcriptome dataset supporting expressed gene discovery in hymenopterans.

Data type / assay

bulk-RNA-seq

Organism

Apis mellifera

Instrument

454 GS FLX

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

2 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 733861889 reported

total reads 1357405 reported

n content pct 0.055 measured

pct q20 bases 87.7 measured

pct q30 bases 70.7 measured

gc content pct 36.8 measured

mean read length 376.1 measured

mean base quality 32.6 measured

adapter content pct 0 measured

duplication rate pct 19 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 54/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 70.7 measured ×1 4%

mean base quality 32.6 measured ×0.6 77%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 19 measured ×0.4 100%

QC cost 14 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0