SRP003261

ENA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

38/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Apis mellifera EST sequencing on 454 GS FLX Titanium yields 6.2M reads with lower Q30 bases (55.7%), capturing expressed-sequence tags for honeybee gene annotation and transcript discovery. 454 read length suits EST assembly; lower accuracy may impair SNP calling, so best reused for qualitative transcript identification and expression-tag mapping.

Data type / assay

bulk-RNA-seq

Organism

Apis mellifera

Instrument

454 GS FLX Titanium

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

6 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3178612952 reported

total reads 6235015 reported

n content pct 0.066 measured

pct q20 bases 79.6 measured

pct q30 bases 55.7 measured

gc content pct 31.7 measured

mean read length 350.2 measured

mean base quality 29.1 measured

adapter content pct 0 measured

duplication rate pct 15.04 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 38/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 55.7 measured ×1 0%

mean base quality 29.1 measured ×0.6 18%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 15.04 measured ×0.4 100%

QC cost 8 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0