SRP003528

ENA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

81/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Apis mellifera (honeybee) EST library from an Illumina Genome Analyzer II with 181 million short reads at 84% Q30. The large transcript survey captures the breadth of bee gene expression across tissues relevant to social behavior.

Data type / assay

bulk-RNA-seq

Organism

Apis mellifera

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

23 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 18181323500 reported

total reads 181813235 reported

n content pct 0.007 measured

pct q20 bases 91.5 measured

pct q30 bases 84.3 measured

gc content pct 37.8 measured

mean read length 100 measured

mean base quality 32.4 measured

adapter content pct 0.31 measured

duplication rate pct 15.67 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 81/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 84.3 measured ×1 72%

mean base quality 32.4 measured ×0.6 73%

adapter content pct 0.31 measured ×0.4 100%

duplication rate pct 15.67 measured ×0.4 100%

QC cost 34 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0