Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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SRP003554

SRA first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Mus musculus bulk RNA-seq from Illumina Genome Analyzer II produces 80M reads with moderate quality (85.1% ≥Q20, 63.7% ≥Q30), supporting tissue-specific and developmental-stage transcriptomics in mice. Moderate depth and quality suit targeted expression studies; well-annotated mouse genome enables direct comparison but limits low-abundance isoform detection.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina Genome Analyzer II
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
10 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 2903392512 reported
total reads 80649792 reported
n content pct 0.764 measured
pct q20 bases 85.1 measured
pct q30 bases 63.7 measured
gc content pct 48.8 measured
mean read length 36 measured
mean base quality 27.2 measured
adapter content pct 0 measured
duplication rate pct 28.34 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 63.7 measured ×1 0%
mean base quality 27.2 measured ×0.6 0%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 28.34 measured ×0.4 100%
QC cost 11 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0