SRP003905

ENA first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

81/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Drosophila melanogaster EST sequencing via 454 GS FLX generating 381 billion bases (96.7% Q20), capturing expressed sequence tags for this model organism at exceptional scale; 454 platform provides longer reads suitable for isoform detection.

Data type / assay

bulk-RNA-seq

Organism

Drosophila melanogaster

Instrument

454 GS FLX

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

85 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 381268600749 reported

total reads 2233062483 reported

n content pct 0.088 measured

pct q20 bases 96.7 measured

pct q30 bases 94.3 measured

gc content pct 53.1 measured

mean read length 76 measured

mean base quality 32.8 measured

adapter content pct 0 measured

duplication rate pct 68.8 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 81/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.3 measured ×1 100%

mean base quality 32.8 measured ×0.6 80%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 68.8 measured ×0.4 14%

QC cost 57 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0