SRP004502

ENA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

53/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Triticum aestivum (wheat) full-length cDNA library from a 454 GS FLX platform with 1.5 million long reads at 71% Q30. The full-length transcripts are valuable for wheat gene annotation and isoform discovery despite moderate sequence quality.

Data type / assay

bulk-RNA-seq

Organism

Triticum aestivum

Instrument

454 GS FLX

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

2 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 748197083 reported

total reads 1469817 reported

n content pct 0.085 measured

pct q20 bases 87.8 measured

pct q30 bases 71.2 measured

gc content pct 46.4 measured

mean read length 355.6 measured

mean base quality 32.8 measured

adapter content pct 0 measured

duplication rate pct 36.8 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 53/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 71.2 measured ×1 6%

mean base quality 32.8 measured ×0.6 80%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 36.8 measured ×0.4 85%

QC cost 33 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0