Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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SRP006382

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

57/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Homo sapiens bulk RNA-seq using Helicos HeliScope single-molecule sequencing yields 433M reads without PCR amplification (43.4% GC content), capturing full-length cDNA profiles without amplification bias or chimera artifacts. Distinctive single-molecule approach avoids amplicon-length bias; limited sequence-index availability and obsolete platform make reanalysis challenging.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Helicos HeliScope
Platform
HELICOS
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
61 runs
Metrics (value · how obtained)
gc sd 11.29 measured
checksum ok yes reported
total bases 14761616771 reported
total reads 433887783 reported
n content pct 0 measured
sampled bases 34418627 measured
sampled reads 1000000 measured
gc content pct 43.6 measured
polyg tail pct 0 measured
read length sd 6.9 measured
quality dropoff 0 measured
read length max 57 measured
read length min 25 measured
read length n50 35 measured
max base quality 14 measured
mean read length 34.4 measured
max n pct per pos 0 measured
mean base quality 14 measured
pct reads lt 100bp 100 measured
read length median 33 measured
adapter content pct 0 measured
median read quality 14 measured
duplication rate pct 4.42 measured
overrepresented top pct 0.25 measured
How this grade was computed
Weighted mean of 3 scored metric(s) → 57/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

mean base quality 14 measured ×0.6 0%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 4.42 measured ×0.4 100%
QC cost 54 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0