SRP015263

ENA first seen 2013

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

57/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Panax ginseng EST sequencing on 454 GS FLX Titanium yields 2.4M reads with moderate quality (89.3% ≥Q20, 71.2% ≥Q30), capturing expressed tags from this medicinal crop plant. 454 read length suits EST assembly for gene-annotation and transcript-discovery; lower Q30 acceptable for qualitative transcript identification and expression-marker design.

Data type / assay

bulk-RNA-seq

Organism

Panax ginseng

Instrument

454 GS FLX Titanium

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

4 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1297829524 reported

total reads 2435951 reported

n content pct 0.024 measured

pct q20 bases 89.3 measured

pct q30 bases 71.2 measured

gc content pct 41.8 measured

mean read length 406.2 measured

mean base quality 33 measured

adapter content pct 0 measured

duplication rate pct 22.62 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 57/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 71.2 measured ×1 6%

mean base quality 33 measured ×0.6 83%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 22.62 measured ×0.4 100%

QC cost 17 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0