SRP016518

ENA first seen 2013

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

74/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Drosophila melanogaster RNA-seq from an Illumina Genome Analyzer with 602 million short reads at 100% Q30, generating 30.1 billion bases. The exceptional quality and depth enable definitive isoform discovery and precise expression quantification in this model organism.

Data type / assay

bulk-RNA-seq

Organism

Drosophila melanogaster

Instrument

Illumina Genome Analyzer

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

43 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 30118639350 reported

total reads 602372787 reported

n content pct 0.028 measured

pct q20 bases 100 measured

pct q30 bases 100 measured

gc content pct 46.1 measured

mean read length 50 measured

mean base quality 30 measured

adapter content pct 0.11 measured

duplication rate pct 56.52 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 74/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%

mean base quality 30 measured ×0.6 33%

adapter content pct 0.11 measured ×0.4 100%

duplication rate pct 56.52 measured ×0.4 41%

QC cost 7 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0