SRP019802

ENA first seen 2013

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

70/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Dromaius novaehollandiae (emu) bulk RNA-seq from HiSeq 2000 generating 3.1 billion bases (88.2% Q20), supporting bird transcriptomics; elevated N-content (1.669%) and reduced Q30 rate (78.9%) indicate moderate quality concerns.

Data type / assay

bulk-RNA-seq

Organism

Dromaius novaehollandiae

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

7 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3079157900 reported

total reads 25559236 reported

n content pct 1.669 measured

pct q20 bases 88.2 measured

pct q30 bases 78.9 measured

gc content pct 48.8 measured

mean read length 70 measured

mean base quality 32.4 measured

adapter content pct 0.02 measured

duplication rate pct 22.94 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 70/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 78.9 measured ×1 45%

mean base quality 32.4 measured ×0.6 73%

adapter content pct 0.02 measured ×0.4 100%

duplication rate pct 22.94 measured ×0.4 100%

QC cost 35 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0