SRP026668

ENA first seen 2013

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

66/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Haemonchus contortus (parasitic nematode) bulk RNA-seq from Genome Analyzer II totaling 37.9 billion bases (92% Q20), enabling comprehensive transcriptomics for this livestock parasite; elevated N-content (0.142%) and moderate Q30 rate (78.1%) suggest some sequence quality trade-offs but acceptable for mapping-based analysis.

Data type / assay

bulk-RNA-seq

Organism

Haemonchus contortus

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

9 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 37870379688 reported

total reads 236267169 reported

n content pct 0.142 measured

pct q20 bases 92 measured

pct q30 bases 78.1 measured

gc content pct 48 measured

mean read length 76 measured

mean base quality 31.8 measured

adapter content pct 1.2 measured

duplication rate pct 21.84 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 66/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 78.1 measured ×1 41%

mean base quality 31.8 measured ×0.6 63%

adapter content pct 1.2 measured ×0.4 100%

duplication rate pct 21.84 measured ×0.4 100%

QC cost 20 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0