SRP027410

ENA first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

68/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Staphylococcus aureus bulk RNA-Seq from HiSeq 2000 with 339M reads at moderate quality (88.1% ≥Q20, 81.4% ≥Q30). Large-scale bacterial transcriptomics dataset supporting gene expression studies in pathogenic gram-positive bacteria.

Data type / assay

bulk-RNA-seq

Organism

Staphylococcus aureus

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

3 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 17301307473 reported

total reads 339241323 reported

n content pct 0.004 measured

pct q20 bases 88.1 measured

pct q30 bases 81.4 measured

gc content pct 38.8 measured

mean read length 51 measured

mean base quality 32.8 measured

adapter content pct 0.01 measured

duplication rate pct 55.17 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 68/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 81.4 measured ×1 57%

mean base quality 32.8 measured ×0.6 80%

adapter content pct 0.01 measured ×0.4 100%

duplication rate pct 55.17 measured ×0.4 44%

QC cost 34 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0