SRP034875

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

64/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Homo sapiens RNA-seq. Adapter contamination dominates: 17.33% (measured, 15/100 score, weight 0.4) indicates incomplete library prep or trimming failure. Q30 82.5% is borderline. Requires aggressive adapter trimming and quality filtering before transcript quantification.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

33 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 348045832744 reported

total reads 1722999172 reported

n content pct 0.003 measured

pct q20 bases 88.6 measured

pct q30 bases 82.5 measured

gc content pct 52.4 measured

mean read length 101 measured

mean base quality 32.6 measured

adapter content pct 17.33 measured

duplication rate pct 25.19 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 64/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.5 measured ×1 63%

mean base quality 32.6 measured ×0.6 77%

adapter content pct 17.33 measured ×0.4 15%

duplication rate pct 25.19 measured ×0.4 100%

QC cost 43 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0