SRP062243

ENA first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

60/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq with catastrophic adapter content (93%) and severe duplication (62.15%) that render this dataset unsuitable for reuse; these metrics indicate the vast majority of sequencing is adapter contamination rather than biological signal.

Data type / assay

bulk-RNA-seq

Organism

Pseudomonas aeruginosa PA14

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

60 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 219784672668 reported

total reads 2176085868 reported

n content pct 0.119 measured

pct q20 bases 93.2 measured

pct q30 bases 84.5 measured

gc content pct 47.7 measured

mean read length 101 measured

mean base quality 34.1 measured

adapter content pct 93 measured

duplication rate pct 62.15 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 60/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 84.5 measured ×1 73%

mean base quality 34.1 measured ×0.6 100%

adapter content pct 93 measured ×0.4 0%

duplication rate pct 62.15 measured ×0.4 29%

QC cost 41 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0