SRP064478

SRA

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

68/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Human transcriptome sequencing via Illumina NextSeq 500 high-throughput platform with 300 bp reads and moderate quality (93.4% Q20), enabling large-scale expression profiling. Quality and read length are suitable for standard gene expression studies, though lower Q20 rates may affect isoform resolution.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

15 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 308762730900 reported

total reads 1029209103 reported

n content pct 0.02 measured

pct q20 bases 93.4 measured

pct q30 bases 85.8 measured

gc content pct 47.4 measured

mean read length 150 measured

mean base quality 33.9 measured

adapter content pct 46.55 measured

duplication rate pct 46.03 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 68/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.8 measured ×1 79%

mean base quality 33.9 measured ×0.6 98%

adapter content pct 46.55 measured ×0.4 0%

duplication rate pct 46.03 measured ×0.4 64%

QC cost 21 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0