SRP065626

ENA first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

34/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Human amplicon with catastrophic adapter contamination at 84.9%—nearly the entire read set consists of adapter—and marginal Q30 at 80.1%. This indicates library failure; unsuitable for reuse.

Data type / assay

amplicon

Organism

Homo sapiens

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

60 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 35712422400 reported

total reads 59520704 reported

n content pct 0 measured

pct q20 bases 85.7 measured

pct q30 bases 80.1 measured

gc content pct 56.6 measured

mean read length 300 measured

mean base quality 32.8 measured

adapter content pct 84.9 measured

duplication rate pct 98.05 measured

mean target coverage 576 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 34/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 80.1 measured ×1 51%

adapter content pct 84.9 measured ×0.5 0%

QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0