SRP069844

ENA first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Mouse targeted amplicon with perfect base quality. Perfect QC (100/100, A) with Q30 of 93.2% and minimal adapter content (0.09%), supporting accurate variant calling at the target locus; the 99.46% duplication is expected and appropriate for focused amplicon design with high coverage (64.5x extrapolated). Excellent for targeted genotyping and mutation detection.

Data type / assay

amplicon

Organism

Mus musculus

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

28 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3485664521 reported

total reads 23083871 reported

n content pct 1.516 measured

pct q20 bases 97.4 measured

pct q30 bases 93.2 measured

gc content pct 37.8 measured

mean read length 151 measured

mean base quality 36 measured

adapter content pct 0.09 measured

duplication rate pct 99.46 measured

mean target coverage 64.5 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.2 measured ×1 100%

adapter content pct 0.09 measured ×0.5 100%

QC cost 36 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0