SRP074110

ENA first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

77/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Human amplicon with moderate quality (Q30=83%) and substantial duplication at 61.65%, the main limitations. Coverage is extrapolated and requires verification; suitable for abundance profiling with bias correction but not for rare variants.

Data type / assay

amplicon

Organism

Homo sapiens

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

83 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 257507839800 reported

total reads 1287539199 reported

n content pct 0.193 measured

pct q20 bases 86.7 measured

pct q30 bases 83 measured

gc content pct 41.1 measured

mean read length 100 measured

mean base quality 32.8 measured

adapter content pct 0.24 measured

duplication rate pct 61.65 measured

mean target coverage 4153.4 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 77/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83 measured ×1 65%

adapter content pct 0.24 measured ×0.5 100%

QC cost 31 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0