SRP099805

ENA first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Targeted human amplicon, Illumina NextSeq 500. A-grade (100/100) with excellent measured metrics: Q30=96.9%, adapters=0%, across 1.4B reads. Duplication=98.98% is expected and acceptable for deep-coverage targeted sequencing. **Caution:** mean_target_coverage (893×) is extrapolated rather than measured, making evidence_strength provisional; validate measured coverage depth independently before committed reuse.

Data type / assay

amplicon

Organism

Homo sapiens

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

14 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 55372788467 reported

total reads 1388193508 reported

n content pct 0.001 measured

pct q20 bases 98.1 measured

pct q30 bases 96.9 measured

gc content pct 46.5 measured

mean read length 19.9 measured

mean base quality 34.3 measured

adapter content pct 0 measured

duplication rate pct 98.98 measured

mean target coverage 893.1 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.9 measured ×1 100%

adapter content pct 0 measured ×0.5 100%

QC cost 16 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0