SRP126669

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

74/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

16S amplicon sequencing of synthetic construct on HiSeq 2000 with >183 million reads and moderate quality (92% Q20, 82.2% Q30), providing positive control data for amplicon pipelines though quality variations may require filtering.

Data type / assay

amplicon

Organism

synthetic construct

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

38 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 70815516354 reported

total reads 183853938 reported

n content pct 0.008 measured

pct q20 bases 92 measured

pct q30 bases 82.2 measured

gc content pct 54.7 measured

mean read length 300 measured

mean base quality 33.8 measured

adapter content pct 0.09 measured

duplication rate pct 87.78 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 74/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.2 measured ×1 61%

adapter content pct 0.09 measured ×0.5 100%

QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0