SRP128749

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

62/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

16S amplicon sequencing of human gut metagenome on MiSeq with >40 million reads and moderate quality (90.8% Q20, 78.5% Q30), offering substantial sample coverage though lower Q30 rates suggest caution with rare OTU calling.

Data type / assay

amplicon

Organism

human gut metagenome

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

746 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 23705070378 reported

total reads 40857196 reported

n content pct 0 measured

pct q20 bases 90.8 measured

pct q30 bases 78.5 measured

gc content pct 53.4 measured

mean read length 289 measured

mean base quality 33 measured

adapter content pct 0 measured

duplication rate pct 89.57 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 62/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 78.5 measured ×1 43%

adapter content pct 0 measured ×0.5 100%

QC cost 3 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0