SRP128925

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

17/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

MicroRNA-seq of plant from HiSeq platform. Grade F with score 17/100—poor quality, unsuitable for reuse. Adapter contamination at 49.97% indicates failed library prep, and both Q30 (46.7%) and mean base quality (24.5) are critically low; this triple failure severely compromises miRNA detection accuracy and species-level identification.

Data type / assay

bulk-RNA-seq

Organism

Arabidopsis thaliana

Instrument

Illumina HiSeq 1000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

115 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 41938505289 reported

total reads 638981034 reported

n content pct 0.007 measured

pct q20 bases 53.3 measured

pct q30 bases 46.7 measured

gc content pct 45.9 measured

mean read length 86 measured

mean base quality 24.5 measured

adapter content pct 49.97 measured

duplication rate pct 25.3 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 17/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 46.7 measured ×1 0%

mean base quality 24.5 measured ×0.6 0%

adapter content pct 49.97 measured ×0.4 0%

duplication rate pct 25.3 measured ×0.4 100%

QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0