SRP133974

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

85/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

454 GS amplicon sequencing of gut metagenome with moderate quality (92.6% Q20) using a legacy sequencing platform; older data useful for historical microbiome composition comparisons and longitudinal microbiota studies.

Data type / assay

amplicon

Organism

gut metagenome

Instrument

454 GS

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

20 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 2103248508 reported

total reads 3502629 reported

n content pct 0.018 measured

pct q20 bases 92.6 measured

pct q30 bases 85.5 measured

gc content pct 53.9 measured

mean read length 300.5 measured

mean base quality 34.5 measured

adapter content pct 0 measured

duplication rate pct 96.35 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 85/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.5 measured ×1 78%

adapter content pct 0 measured ×0.5 100%

QC cost 16 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0