SRP141144

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

95/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

16S amplicon sequencing from Mus musculus on NextSeq 550 with >626 million reads and perfect quality (100% Q20, 100% Q30), representing exceptional over-sequencing with zero quality filtering needs suitable for stringent analytical pipelines.

Data type / assay

amplicon

Organism

Mus musculus

Instrument

NextSeq 550

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

37 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 65778457994 reported

total reads 626145432 reported

n content pct 0.002 measured

pct q20 bases 100 measured

pct q30 bases 100 measured

gc content pct 52.2 measured

mean read length 90 measured

mean base quality 30 measured

adapter content pct 2.96 measured

duplication rate pct 97.81 measured

mean target coverage 1218.1 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 95/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%

adapter content pct 2.96 measured ×0.5 86%

QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0