SRP142550

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

98/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

16S amplicon sequencing of gut metagenome on 454 GS FLX with ~954k reads and good quality (96.7% Q20, 89.3% Q30), representing data from an older pyrosequencing platform with inherent longer read lengths but lower per-base accuracy.

Data type / assay

amplicon

Organism

gut metagenome

Instrument

454 GS FLX

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

393 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 325838280 reported

total reads 953760 reported

n content pct 0.006 measured

pct q20 bases 96.7 measured

pct q30 bases 89.3 measured

gc content pct 56.1 measured

mean read length 297 measured

mean base quality 37.1 measured

adapter content pct 0 measured

duplication rate pct 87.06 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 98/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 89.3 measured ×1 97%

adapter content pct 0 measured ×0.5 100%

QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0