SRP145574

SRA

Provenance — who produced it, who reused it

Linked to 0 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

No linked papers found in the corpus yet.

Deep data QC

97/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Large Drosophila bulk-RNA-seq (491M reads, 147B bases) with extended reads (150bp) and overall excellent quality; Q30 at 88.4% is slightly lower than peak-quality datasets but compensated by high read depth and length, supporting complex transcriptome analysis.

Data type / assay

bulk-RNA-seq

Organism

Drosophila melanogaster

Instrument

Illumina HiSeq 4000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

32 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 147458121000 reported

total reads 491527070 reported

n content pct 0.001 measured

pct q20 bases 94.9 measured

pct q30 bases 88.4 measured

gc content pct 46 measured

mean read length 150 measured

mean base quality 37.3 measured

adapter content pct 0.36 measured

duplication rate pct 27.61 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 97/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 88.4 measured ×1 92%

mean base quality 37.3 measured ×0.6 100%

adapter content pct 0.36 measured ×0.4 100%

duplication rate pct 27.61 measured ×0.4 100%

QC cost 19 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0