SRP148115

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

74/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

RAD-Seq from Gasterosteus aculeatus (threespine stickleback) on HiSeq 2500 with >1.5 billion reads and good quality (89.8% Q20, 82.1% Q30), providing genome-wide SNP discovery across populations with moderate coverage.

Data type / assay

amplicon

Organism

Gasterosteus aculeatus

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

970 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 143554551680 reported

total reads 1511100544 reported

n content pct 0.135 measured

pct q20 bases 89.8 measured

pct q30 bases 82.1 measured

gc content pct 50.3 measured

mean read length 95 measured

mean base quality 32.7 measured

adapter content pct 0.09 measured

duplication rate pct 78.62 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 74/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.1 measured ×1 61%

adapter content pct 0.09 measured ×0.5 100%

QC cost 30 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0