SRP148357

ENA first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

67/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

16S amplicon sequencing of soil metagenome on MiSeq with ~329k reads and good quality (96.4% Q20, 91.4% Q30), enabling soil microbiota profiling with solid taxonomic resolution for ecological studies.

Data type / assay

amplicon

Organism

soil metagenome

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

9 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 198049572 reported

total reads 328986 reported

n content pct 0 measured

pct q20 bases 96.4 measured

pct q30 bases 91.4 measured

gc content pct 45.8 measured

mean read length 301 measured

mean base quality 35.8 measured

adapter content pct 50.26 measured

duplication rate pct 99.27 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 67/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91.4 measured ×1 100%

adapter content pct 50.26 measured ×0.5 0%

QC cost 2 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0