SRP160000

SRA

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Large-scale transcriptome sequencing of soybean (*Glycine max*) using Illumina HiSeq 2500 with excellent quality metrics (98.8% Q20, 96.1% Q30) and substantial depth (281M reads). Enables comprehensive gene expression analysis and splice-variant discovery relevant to crop genetics and developmental studies.

Data type / assay

bulk-RNA-seq

Organism

Glycine max

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

8 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 74220552450 reported

total reads 281012034 reported

n content pct 0 measured

pct q20 bases 98.8 measured

pct q30 bases 96.1 measured

gc content pct 54.8 measured

mean read length 150 measured

mean base quality 39.5 measured

adapter content pct 0 measured

duplication rate pct 90.25 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.1 measured ×1 100%

mean base quality 39.5 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 90.25 measured ×0.4 0%

QC cost 15 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0