SRP164394

ENA first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

17/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq from Bifidobacterium breve on MiSeq with ~34.9 million reads but poor quality (71.9% Q20, 65% Q30), suitable for basic transcriptome profiling in this probiotic bacterium with acknowledgment of quality limitations.

Data type / assay

bulk-RNA-seq

Organism

Bifidobacterium breve

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

6 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 2594276447 reported

total reads 34906541 reported

n content pct 0.005 measured

pct q20 bases 71.9 measured

pct q30 bases 65 measured

gc content pct 73.2 measured

mean read length 74.3 measured

mean base quality 28.2 measured

adapter content pct 0 measured

duplication rate pct 76.56 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 17/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 65 measured ×1 0%

mean base quality 28.2 measured ×0.6 3%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 76.56 measured ×0.4 0%

QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0