SRR002062

ENA first seen 2012

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

EST bulk RNA-seq from Saccharomyces cerevisiae on Illumina Genome Analyzer with ~3.4 million reads but poor quality (48.4% Q20, 40.6% Q30) and high N-content (2.8%), suggesting early/problematic sequencing run.

Data type / assay

bulk-RNA-seq

Organism

Saccharomyces cerevisiae

Instrument

Illumina Genome Analyzer

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 120562890 reported

total reads 3444654 reported

n content pct 2.814 measured

pct q20 bases 48.4 measured

pct q30 bases 40.6 measured

gc content pct 41 measured

mean read length 35 measured

mean base quality 21.1 measured

adapter content pct 0.21 measured

duplication rate pct 8.95 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 40.6 measured ×1 0%

mean base quality 21.1 measured ×0.6 0%

adapter content pct 0.21 measured ×0.4 100%

duplication rate pct 8.95 measured ×0.4 100%

QC cost 17 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0