SRR006428

ENA first seen 2012

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

29/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Whole-exome capture on legacy 454 platform. Grade F with score 29/100; provisional reading pending measured confirmation. Both Q30 (46.8%) and extrapolated coverage (12×) are critically deficient—too shallow for variant calling and too low-accuracy for exome analysis; evidence_strength=0.61 reflects reliance on extrapolated coverage requiring deep measured revalidation.

Data type / assay

WES

Organism

Homo sapiens

Instrument

454 GS FLX

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 745915308 reported

total reads 1266358 reported

n content pct 0.047 measured

pct q20 bases 78.6 measured

pct q30 bases 46.8 measured

gc content pct 43 measured

mean read length 303 measured

mean base quality 26.5 measured

adapter content pct 0 measured

duplication rate pct 6.24 measured

mean target coverage 12 extrapolated

How this grade was computed

Weighted mean of 4 scored metric(s) → 29/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published WES thresholds, weighted by its importance; nothing is hidden or subjective.

mean target coverage 12 extrapolated ×1.2 0%

pct q30 bases 46.8 measured ×1 0%

duplication rate pct 6.24 measured ×0.5 100%

adapter content pct 0 measured ×0.4 100%

QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0