Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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SRR034786

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

31/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Drosophila pseudoobscura bulk RNA-seq with complete base quality failure (Grade F, 31/100). Zero percent of bases pass Q30 and mean quality is only 23.2—both fail completely—rendering the dataset unsuitable for standard expression or variant analysis. Not suitable for reuse without re-sequencing validation.

Data type / assay
bulk-RNA-seq
Organism
Drosophila pseudoobscura
Instrument
Illumina Genome Analyzer
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
gc sd 11.53 measured
checksum ok yes reported
total bases 105392844 reported
total reads 2927579 reported
n content pct 0.124 measured
pct q20 bases 78 measured
pct q30 bases 0 measured
pct reads q30 0 measured
sampled bases 36000000 measured
sampled reads 1000000 measured
gc content pct 43.8 measured
polyg tail pct 0.01 measured
read length sd 0 measured
quality dropoff -9.1 measured
read length max 36 measured
read length min 36 measured
read length n50 36 measured
max base quality 35 measured
mean read length 36 measured
max n pct per pos 0.345 measured
mean base quality 23.1 measured
pct reads lt 100bp 100 measured
read length median 36 measured
adapter content pct 0.01 measured
median read quality 23.6 measured
duplication rate pct 35.59 measured
overrepresented top pct 0.12 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 31/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 0 measured ×1 0%
mean base quality 23.1 measured ×0.6 0%
adapter content pct 0.01 measured ×0.4 100%
duplication rate pct 35.59 measured ×0.4 88%
QC cost 23 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0