SRR037513

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Drosophila mojavensis RNA-seq, short-read EST. Poor base quality: Q30 51.4% and mean_base_quality 25.4 (both 0/100 score, measured)—borderline reads at 36bp. Mapping will suffer; use only for high-abundance transcripts or as validation cohort, not for quantitative expression profiling.

Data type / assay

bulk-RNA-seq

Organism

Drosophila mojavensis

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 113945256 reported

total reads 3165146 reported

n content pct 1.525 measured

pct q20 bases 80.2 measured

pct q30 bases 51.4 measured

gc content pct 39.4 measured

mean read length 36 measured

mean base quality 25.4 measured

adapter content pct 0 measured

duplication rate pct 19.52 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 51.4 measured ×1 0%

mean base quality 25.4 measured ×0.6 0%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 19.52 measured ×0.4 100%

QC cost 21 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0