SRR042454

ENA first seen 2012

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

19/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read bulk RNA-seq from mouse. Grade F; poor quality across all dimensions—Q30 44.1%, mean base quality 25.3, and duplication 69.22%—indicating degraded library and/or sequencing failure on Genome Analyzer II. Very short reads (32 bp) further limit mapping utility. Not suitable for expression analysis; reuse not recommended.

Data type / assay

bulk-RNA-seq

Organism

Mus musculus

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 141326496 reported

total reads 4416453 reported

n content pct 0.354 measured

pct q20 bases 60.5 measured

pct q30 bases 44.1 measured

gc content pct 47.1 measured

mean read length 32 measured

mean base quality 25.3 measured

adapter content pct 0 measured

duplication rate pct 69.22 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 19/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 44.1 measured ×1 0%

mean base quality 25.3 measured ×0.6 0%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 69.22 measured ×0.4 13%

QC cost 19 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0