SRR042467

ENA first seen 2012

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

17/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk RNA-seq from Mus musculus with severely compromised quality (51.2% Q30 bases, mean quality 27.3) and near-complete duplication (87.5%) from an older Genome Analyzer II platform, rendering it unsuitable for reliable expression analysis; strongly recommend against reuse.

Data type / assay

bulk-RNA-seq

Organism

Mus musculus

Instrument

Illumina Genome Analyzer II

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 166341968 reported

total reads 6397768 reported

n content pct 0.019 measured

pct q20 bases 66.8 measured

pct q30 bases 51.2 measured

gc content pct 46.5 measured

mean read length 26 measured

mean base quality 27.3 measured

adapter content pct 0 measured

duplication rate pct 87.53 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 17/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 51.2 measured ×1 0%

mean base quality 27.3 measured ×0.6 0%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 87.53 measured ×0.4 0%

QC cost 17 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0