SRR053821

ENA first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

41/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Amplicon sequencing (454 GS FLX, long-read) from unidentified mixed DNA library. Grade F; Q30 72.2% is below amplicon thresholds, and 98.42% duplication is artificial—likely single-molecule collapse or over-clustering rather than true biological duplication. Unknown taxonomy and quality metrics limit reusability.

Data type / assay

amplicon

Organism

unidentified;mixed DNA library

Instrument

454 GS FLX

Platform

LS454

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 26834893 reported

total reads 52725 reported

n content pct 0 measured

pct q20 bases 90.5 measured

pct q30 bases 72.2 measured

gc content pct 53.1 measured

mean read length 462.6 measured

mean base quality 33.3 measured

adapter content pct 0 measured

duplication rate pct 98.42 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 41/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 72.2 measured ×1 11%

adapter content pct 0 measured ×0.5 100%

QC cost 2 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0